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Protocoles pour la mutagenèse CRISPR/Cas9 de la mouche orientale des fruits Bactrocera dorsalis
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Protocoles pour la mutagenèse CRISPR/Cas9 de la mouche orientale des fruits Bactrocera dorsalis

Article DOI: 10.3791/64195-v • 08:19 min • September 28th, 2022
September 28th, 2022 •
1, 1, 1, 1, 2, 1, 2
1Key Laboratory of Sustainable Forest Ecosystem Management - Ministry of Education, Northeast Forestry University, 2Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences

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Cet article présente les protocoles étape par étape pour la mutagénèse CRISPR / Cas9 de la mouche orientale des fruits Bactrocera dorsalis. Les étapes détaillées fournies par ce protocole normalisé serviront de guide utile pour générer des mouches mutantes pour les études de gènes fonctionnels chez B. dorsalis.

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Biologie numéro 187
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