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JoVE Journal Biology
Combinatie van 3D Magnetic Force Actuator en multifunctionele fluorescentie beeldvorming om Nucleus Mechanobiology te bestuderen
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Combinatie van 3D Magnetic Force Actuator en multifunctionele fluorescentie beeldvorming om Nucleus Mechanobiology te bestuderen

Article DOI: 10.3791/64098-v • 06:54 min • July 5th, 2022
July 5th, 2022 •
1,5, 1,5, 1, 1, 2, 3,5, 4, 5,6,7, 1, 1,5,8,9
1Department of Mechanical and Aerospace Engineering, Herbert Wertheim College of Engineering, University of Florida, 2Department of Materials Science and Engineering, University of Florida, 3Department of Biostatistics, University of Florida, 4Department of Biomedical Engineering, College of Engineering (COE), University of Delaware (UD), 5UF Health Cancer Center, University of Florida, 6Department of Physics, College of Liberal Arts and Sciences, University of Florida, 7Department of Anatomy and Cell Biology, College of Medicine, University of Florida, 8J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, 9Department of Physiology and Functional Genomics, University of Florida

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Deze studie presenteert een nieuw protocol om direct mechanische kracht uit te oefenen op de celkern door middel van magnetische microbeads die in het cytoplasma worden afgeleverd en om gelijktijdige live-cell fluorescerende beeldvorming uit te voeren.

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Biologie Nummer 185 Nucleus mechanobiologie magnetische kracht CRISPR/Cas9 mechanotransductie zachte actieve materie ja-geassocieerd eiwit (YAP) fluorescerende functionele beeldvorming digitale beeldvormingsverwerking optogenetica epigenetica
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