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Journal / Biology / 抗原タグ付きブロッキング病変とのレプリソーム遭遇の可視化…
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抗原タグ付きブロッキング病変とのレプリソーム遭遇の可視化
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抗原タグ付きブロッキング病変とのレプリソーム遭遇の可視化

Article DOI: 10.3791/61689-v • 08:24 min • July 27th, 2021
July 27th, 2021 •
*1, *2, 1, 3, 1, 4, 5, 6, 1, 1
1Laboratory of Molecular Gerontology, National Institute on Aging, National Institutes of Health, 2Institute of Chemical Biology and Nanomedicine, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biology, Hunan University, 3Department of Molecular Biology and Genetics, Cornell University, 4Department of Cellular and Molecular Medicine, University of Copenhagen, 5Horizon Discovery, 6Boston University School of Medicine
* These authors contributed equally

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DNA付加体との複製フォーク衝突は二本鎖切断を誘発する可能性がありますが、レプリソームとブロッキング病変の間の相互作用についてはあまり知られていません。近接ライゲーションアッセイを使用して、これらの遭遇を視覚化し、レプリソーム組成の結果を特徴付けました。

Tags

生物学、第173号、レプリソーム、ブロッキング病変、PLA、近接ライゲーションアッセイ、DNA鎖間架橋、抗原タグ付き架橋
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